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KMID : 0894520120160020137
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Development & Reproduction
2012 Volume.16 No. 2 p.137 ~ p.143
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Effect of HCG, LHRHa, Ovaprim and Pimozide on Ovulation Induction in Long Snout Bullhead Leiocassis longirotris
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Lim Sang-Gu
Kim Kwang-Suk
Kim Young-Soo
Han Hyung-Gyun
Kim Eung-Oh
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Abstract
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The main objective of this study is to investigate the effects of ovulation induction by treating HCG, LHRHa, GnRHa, ovaprim and pimozidein in long snout bullhead, L. longriostris. All hormons were injected into the muscles of back. Concentration of LHRHa to injection were 20, 50, 100, 150 and 200 ug/kg and same concentration of LHRHa was injected after 24 hour. The ovulation induction rate was 100% and fertilization and hatching rates were 68.4, 45.2, 58.4 and 33.6% in 50 and 100 ug/kg. The times to ovulation were between 28 and 44 h. HCG was injected in long snout bullhead at 5,000, 10,000, 15,000, 20,000 and 25,000 IU/kg. The ovulation induction rate was 50% in 15,000 and 20,000 IU/kg. Fertlilzation and hatching rates were 55.2, 45.6, 52.8 and 44.8%. Ovulation time was between 72~80 h. HCG concentration of 500, 1,000, 2,000 and 4,000 IU/kg were injected with 50ug of LHRHa. Ovulation and hatching rates in 2,000 IU/kg were 75 and 35%. Ovulation time was 28~48 h. Ovaprim of 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mL/kg were injected to the abdominal cavity. The ovulation induction rate was highest at 2.5 mL/kg to 50% and ovulation time was between 66~86 h. LHRHa concentration of 50, 100, 200, 300 and 400 ug/kg was injected with pimozide (1,000u}g/kg). Ovulation induction rate was 87% at 100 and 200 IU/kg with pimozide. Ovulation time was between 66~86 h.
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KEYWORD
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Long snout bullhead, L. longriostris, HCG, LHRHa, Ovaprim, Pimozide, Ovualation induction, Ovulation time
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